FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAADRAAPEI-206_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAADRAAPEI-206_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39591655
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGTTCCTGGTGCTCCTTGGAGCTGCCTTTGCTCTGGATGATGAC578290.1460636086064096No Hit
CACCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGGC532500.13449804005414778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG78300.021.295464
AGAATCG85700.019.6874873
AATCGGT84200.019.6203825
ACGCGTA32300.016.5493822
ATCGGTT107800.015.8351636
GTTCAAT102100.015.1282591
TTCAATC123000.014.3858752
CGGTTTT115650.014.3418558
CTTACCC76000.014.3136371
GAGTTAA71650.013.8156743
TGCCGTA110550.013.73060531
TCGGTTT128350.013.6083117
CACGCGT40400.013.2313121
CGGCGAT83850.013.210441
CAATCGG20550.013.1665764
ACGATCC38300.013.1526253
CAAGAAT154750.012.8900441
ATTGGCG183050.012.8345646
TGAGTTA78850.012.783482
CCGTAAA117650.012.71528833
Produced by FastQC (version 0.11.5)